Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] in Western Blot (Human)
Addgene #211757
- Data Submitted By
- Amrita Rhoads and Katherine Nardone
- Lab Name
- Addgene
- Submission Date
- March 20, 2024
- Publication Date
- March 20, 2024 (modified April 05, 2024)
- Abstract
- Experimental results for Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] (Addgene Antibody 211757) in a western blot assay with a human sample.
Image attribution: Amrita Rhoads, Katherine Nardone
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86]
Addgene #211757 -
goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078
Materials & Methods
Sample
- Species
- Human
- Cell / Tissue Type
- HeLa
Buffer
- Name
- TBS-T
- pH
- 7.4
Blocking
- Agent
- Milk
- Concentration
- 5%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86]
- Source
- Addgene
- Catalog Number
- 211757
- Species
- Mouse
- Gene / Target
- N
- Concentration
- 5 µg/mL
- Time
- overnight
- Temperature
- 4 °C
Secondary Antibody
- Name
- goat anti-mouse IgG (H+L) HRP
- Source
- Thermo Fisher
- Catalog Number
- A16078
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
Cell lysates from HeLa cells transduced with 141391-LV or 141386-LV were reduced and separated by denaturing SDS-PAGE
- Protocol
- Addgene Western Blot protocol
- Predicted Molecular Weight
- 49 kDa
Results
- Result
- Pass (The antibody worked under these conditions)