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Anti-KChIP2b K+ channel [K60/73R] in Western Blot (Rat)

Addgene #182226

Data Submitted By
James Trimmer
Lab Name
James Trimmer, UC Davis
Submission Date
February 06, 2024
Publication Date
February 06, 2024 (modified February 06, 2024)
Abstract
Experimental results for Anti-KChIP2b K+ channel [K60/73R] (Addgene Antibody 182226) in a western blot assay with a rat sample.
Caption: Crude rat membranes immunoblotted against Anti-KChIP2b K+ channel hybridoma-derived antibody from the Trimmer Lab at UC Davis (H) or purified recombinant antibody produced at Addgene (R).
Image attribution: James Trimmer, UC Davis
Caption: Crude rat membranes immunoblotted against Anti-KChIP2b K+ channel [K60/73R] hybridoma-derived antibody from the Trimmer Lab at UC Davis (H) or purified recombinant antibody produced at Addgene (R).
Image attribution: James Trimmer, UC Davis

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used
Anti-KChIP2b K+ channel [K60/73R]
Addgene #182226
Mix of HRP-conjugated goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgG2b
Jackson Labs #

Materials & Methods

Sample

Species
Rat
Cell / Tissue Type
Brain

Buffer

Name
TBS
pH
7.5

Blocking

Agent
Milk
Concentration
4%
Time
45 min
Temperature
Room Temperature

Primary Antibody

Name
Anti-KChIP2b K+ channel [K60/73R]
Source
Addgene
Catalog Number
182226
Species
Mouse
Gene / Target
Kcnip2
Concentration
5 ยตg/mL
Time
45 min
Temperature
Room Temperature
View in Addgene Catalog

Secondary Antibody

Name
Mix of HRP-conjugated goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgG2b
Source
Jackson Labs
Concentration
1:8333
Time
45 min
Temperature
Room Temperature

Additional Information

Additional Information

Total rat brain extract was reduced and separated by denaturing SDS-PAGE

NeuroMab hybridoma clone datasheet (Link opens in a new window)

Protocol
Trimmer lab immunoblot labeling protocol (Link opens in a new window)
Predicted Molecular Weight
35 kDa

Results

Result
Pass (The antibody worked under these conditions)