Anti-Mortalin/GRP75 [N52A/42.1R] in Immunohistochemistry (Rat)
Addgene #180091
- Data Submitted By
- James Trimmer
- Lab Name
- James Trimmer, UC Davis
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified February 06, 2024)
- Abstract
- Experimental results for Anti-Mortalin/GRP75 [N52A/42.1R] (Addgene Antibody 180091) in an immunohistochemistry assay with a rat sample.
![Free-floating rat brain sections were fixed and stained with purified Anti-Mortalin/GRP75 [N52A/42.1R] or hybridoma-derived tissue culture supernatant. The staining patterns from antibody produced at Addgene (AR0014) were compared to UC Davis-derived antibody (TC Supernatant).](https://datahub-media.addgene.org/report-images/ph0xgc/180091-Immunohistochemistry-2022-05-20_114921.081768.png)
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Mortalin/GRP75 [N52A/42.1R]
Addgene #180091 -
mix of biotinylated goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgG2b
Jackson Labs #115-065-205, 115-065-206, 115-065-207
Materials & Methods
Sample
- Species
- Rat
- Cell / Tissue Type
- Brain
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
Permeabilization / Delipidation
- Agent
- PBS containing 1:2000 avidin and 0.5% Triton X-100
- Time
- 45 min
- Temperature
- Room Temperature
Blocking
- Agent
- Serum
- Concentration
- 0.5%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Mortalin/GRP75 [N52A/42.1R]
- Source
- Addgene
- Catalog Number
- 180091
- Species
- Mouse
- Gene / Target
- Hspa9
- Concentration
- 0.4 µg/mL
- Time
- overnight
- Temperature
- 4 °C
Secondary Antibody
- Name
- mix of biotinylated goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgG2b
- Source
- Jackson Labs
- Catalog Number
- 115-065-205, 115-065-206, 115-065-207
- Concentration
- 0.5 µg/mL
- Time
- 1 hour
- Temperature
- Room Temperature
Additional Information
- Additional Information
Tissue type: Free-floating brain sections
Antigen-retrieval: No
- Protocol
- Trimmer lab immunohistochemistry protocol (Link opens in a new window)
Results
- Result
- Pass (The antibody worked under these conditions)