Anti-Fibronectin [G4] in Western Blot
Addgene #203879
- Data Submitted By
- Meghan Rego
- Lab Name
- Addgene
- Submission Date
- March 20, 2024
- Publication Date
- March 20, 2024 (modified April 05, 2024)
- Abstract
- Experimental results for Anti-Fibronectin [G4] (Addgene Antibody 203879) in a western blot assay.
Image attribution: Meghan Rego
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Fibronectin [G4]
Addgene #203879 -
goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078
Materials & Methods
Sample
- Species
- Other
- Cell / Tissue Type
- Purified Protein
Buffer
- Name
- TBS-T
- pH
- 7.4
Blocking
- Agent
- Milk
- Concentration
- 5%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Fibronectin [G4]
- Source
- Addgene
- Catalog Number
- 203879
- Species
- Mouse
- Gene / Target
- FN1
- Concentration
- 1 µg/mL
- Time
- 45 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- goat anti-mouse IgG (H+L) HRP
- Source
- Thermo Fisher
- Catalog Number
- A16078
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
Purified recombinant 6xHIS-Fibronectin or 7xHIS-RAN-RFP2 was reduced and separated by denaturing SDS-PAGE
- Protocol
- Addgene Western Blot protocol
- Predicted Molecular Weight
- 270.1 kDa
Results
- Result
- Pass (The antibody worked under these conditions)