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Rabbit IgG Isotype Control [IPI-MOPC-21] in Surface Plasmon Resonance (SPR)

Addgene #237822

Data Submitted By
Murali Anuganti , Aditi Kachare , Travis Riedel and Deborah Moshinsky
Lab Name
Institute for Protein Innovation (IPI)
Submission Date
March 25, 2026
Publication Date
March 27, 2026 (modified April 14, 2026)
DOI
https://doi.org/10.57733/addgene.fwu3v3
Abstract
Experimental results for Rabbit IgG Isotype Control [IPI-MOPC-21] (Addgene #237822) in SPR assay with a mouse sample.
Citation
Rabbit IgG Isotype Control [IPI-MOPC-21] in Surface Plasmon Resonance (SPR). Anuganti M, Kachare A, Riedel T, Moshinsky D 2026. Addgene Report, https://doi.org/10.57733/addgene.fwu3v3
Surface Plasmon Resonance (SPR) kinetics analysis of the interaction between Rabbit IgG Isotype Control [IPI-MOPC-21] and mouse NRXN2a. SPR binding kinetics were measured on a Carterra LSA using HC30M chips (Carterra, cat. #4279) at 25 °C. A) Anti-Neurexin-2-alpha [IPI-mNRXN2a.10] (Addgene #237806) and B) IPI-MOPC-21 (Addgene #237822) were captured using goat anti-rabbit IgG Fc (Jackson ImmunoResearch, cat. #111-005-046) immobilized by amine coupling. The mouse NRXN2a antigen (400 nM with five 3-fold dilutions) was injected in standard antigen buffer (20 mM HEPES pH 7.4, 150 mM NaCl, 1 mM CaCl₂, 1 mM MgCl₂, 0.005% Tween 80) with 300 s association and dissociation phases followed by acid regeneration. Data (reference/buffer subtracted, smoothed) were globally fit to a 1:1 Langmuir model to derive ka, kd, and KD using Carterra Kinetics software v1.9.2.44.63, and replotted in OriginPro 2023b. IPI-mNRXN2a.10 produced a clear binding response consistent with a premium binding profile, whereas IPI-MOPC-21 showed no binding as expected and served as a negative control.
Caption: Surface Plasmon Resonance (SPR) kinetics analysis of the interaction between Rabbit IgG Isotype Control [IPI-MOPC-21] and mouse NRXN2a. SPR binding kinetics were measured on a Carterra LSA using HC30M chips (Carterra, cat. #4279) at 25 °C. A) Anti-Neurexin-2-alpha [IPI-mNRXN2a.10] (Addgene #237806) and B) IPI-MOPC-21 (Addgene #237822) were captured using goat anti-rabbit IgG Fc (Jackson ImmunoResearch, cat. #111-005-046) immobilized by amine coupling. The mouse NRXN2a antigen (400 nM with five 3-fold dilutions) was injected in standard antigen buffer (20 mM HEPES pH 7.4, 150 mM NaCl, 1 mM CaCl₂, 1 mM MgCl₂, 0.005% Tween 80) with 300 s association and dissociation phases followed by acid regeneration. Data (reference/buffer subtracted, smoothed) were globally fit to a 1:1 Langmuir model to derive ka, kd, and KD using Carterra Kinetics software v1.9.2.44.63, and replotted in OriginPro 2023b. IPI-mNRXN2a.10 produced a clear binding response consistent with a premium binding profile, whereas IPI-MOPC-21 showed no binding as expected and served as a negative control.
Image attribution: Institute for Protein Innovation (IPI)

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used
Rabbit IgG Isotype Control [IPI-MOPC-21]
Addgene #237822
AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific
Jackson ImmunoResearch #111-005-046

Materials & Methods

Application Type
Surface Plasmon Resonance (SPR)

Sample

Target Species
Mouse

Primary Antibody

Name
Rabbit IgG Isotype Control [IPI-MOPC-21]
Source
Addgene
Catalog Number
237822
Host Species
Rabbit
Concentration
0.25 µg/mL (1:4,000 dilution)
Temperature
Room Temperature
View in Addgene Catalog

Secondary Antibody

Name
AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific
Source
Jackson ImmunoResearch
Catalog Number
111-005-046
Concentration
Immobilized by amine coupling

Results

Result
Pass (The antibody worked under these conditions)
Describe Results
IPI-MOPC-21 showed no binding as expected and served as a negative control