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Rabbit IgG Isotype Control [IPI-MOPC-21] in Surface Plasmon Resonance (SPR)

Addgene #237822

Data Submitted By
Murali Anuganti , Aditi Kachare , Travis Riedel and Deborah Moshinsky
Lab Name
Institute for Protein Innovation (IPI)
Submission Date
March 25, 2026
Publication Date
March 27, 2026 (modified April 13, 2026)
Abstract
Experimental results for Rabbit IgG Isotype Control [IPI-MOPC-21] (Addgene #237822) in SPR assay with a mouse sample.
Surface Plasmon Resonance (SPR) kinetics analysis of the interaction between Rabbit IgG Isotype Control [IPI-MOPC-21] and mouse NRXN2a. SPR binding kinetics were measured on a Carterra LSA using HC30M chips (Carterra, cat. #4279) at 25 °C. A) Anti-Neurexin-2-alpha [IPI-mNRXN2a.10] (Addgene #237806) and B) IPI-MOPC-21 (Addgene #237822) were captured using goat anti-rabbit IgG Fc (Jackson ImmunoResearch, cat. #111-005-046) immobilized by amine coupling. The mouse NRXN2a antigen (400 nM with five 3-fold dilutions) was injected in standard antigen buffer (20 mM HEPES pH 7.4, 150 mM NaCl, 1 mM CaCl₂, 1 mM MgCl₂, 0.005% Tween 80) with 300 s association and dissociation phases followed by acid regeneration. Data (reference/buffer subtracted, smoothed) were globally fit to a 1:1 Langmuir model to derive ka, kd, and KD using Carterra Kinetics software v1.9.2.44.63, and replotted in OriginPro 2023b. IPI-mNRXN2a.10 produced a clear binding response consistent with a premium binding profile, whereas IPI-MOPC-21 showed no binding as expected and served as a negative control.
Caption: Surface Plasmon Resonance (SPR) kinetics analysis of the interaction between Rabbit IgG Isotype Control [IPI-MOPC-21] and mouse NRXN2a. SPR binding kinetics were measured on a Carterra LSA using HC30M chips (Carterra, cat. #4279) at 25 °C. A) Anti-Neurexin-2-alpha [IPI-mNRXN2a.10] (Addgene #237806) and B) IPI-MOPC-21 (Addgene #237822) were captured using goat anti-rabbit IgG Fc (Jackson ImmunoResearch, cat. #111-005-046) immobilized by amine coupling. The mouse NRXN2a antigen (400 nM with five 3-fold dilutions) was injected in standard antigen buffer (20 mM HEPES pH 7.4, 150 mM NaCl, 1 mM CaCl₂, 1 mM MgCl₂, 0.005% Tween 80) with 300 s association and dissociation phases followed by acid regeneration. Data (reference/buffer subtracted, smoothed) were globally fit to a 1:1 Langmuir model to derive ka, kd, and KD using Carterra Kinetics software v1.9.2.44.63, and replotted in OriginPro 2023b. IPI-mNRXN2a.10 produced a clear binding response consistent with a premium binding profile, whereas IPI-MOPC-21 showed no binding as expected and served as a negative control.
Image attribution: Institute for Protein Innovation (IPI)

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used
Rabbit IgG Isotype Control [IPI-MOPC-21]
Addgene #237822
AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific
Jackson ImmunoResearch #111-005-046

Materials & Methods

Application Type
Surface Plasmon Resonance (SPR)

Sample

Target Species
Mouse

Primary Antibody

Name
Rabbit IgG Isotype Control [IPI-MOPC-21]
Source
Addgene
Catalog Number
237822
Host Species
Rabbit
Concentration
0.25 µg/mL (1:4,000 dilution)
Temperature
Room Temperature
View in Addgene Catalog

Secondary Antibody

Name
AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific
Source
Jackson ImmunoResearch
Catalog Number
111-005-046
Concentration
Immobilized by amine coupling

Results

Result
Pass (The antibody worked under these conditions)
Describe Results
IPI-MOPC-21 showed no binding as expected and served as a negative control