Anti-Kvbeta1.2/KCNAB1 K+ channel [K47/42R] in Western Blot (Rat)

Hybridoma

Data Submitted By: James Trimmer
Lab Name: UC Davis/NIH NeuroMab Facility
Submission Date: March 20, 2024
Publication Date: October 16, 2024 (modified October 21, 2024)
Abstract: Experimental results for Anti-Kvbeta1.2/KCNAB1 K+ channel [K47/42R] (hybridoma-derived) in a western blot assay with a rat sample.

Adult rat brain membrane immunoblot — Caption: Adult rat brain membrane immunoblot
Image attribution: UC Davis/NIH NeuroMab Facility

Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus
[HBM(H)] and probed with K37/89 (left) or N52A/42 (right) TC
supe. — Caption: Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with K37/89 (left) or N52A/42 (right) TC supe.
Image attribution: UC Davis/NIH NeuroMab Facility

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-Kvbeta1.2/KCNAB1 K+ channel [K47/42R]
Addgene #209588

Materials & Methods

Sample

Species: Rat
Cell / Tissue Type: Brain

Primary Antibody

Name: Anti-Kvbeta1.2/KCNAB1 K+ channel [K47/42R]
Species: Mouse
Gene / Target: KCNAB1

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)
Protocol: Trimmer lab immunoblot labeling protocol (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)