pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA in Mouse (AAV Retrograde)
Addgene #20298-AAVrg
- Data Submitted By
- J. Elliott Robinson and Viviana Gradinaru
- Submission Date
- June 25, 2019
- Publication Date
- June 25, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: Gerry Coughlin
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Addgene #20298-AAVrg
Virus & Injection
Virus
- Virus Name
- pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 20298-AAVrg
- Promoter
- EF1a
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- 200 nl bilaterally
- Injection Rate
- 20 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Median eminence, NeuroNames: 402
- Additional Details
- GnRH neurons in the median eminence.
Other Details
- Species
- Mouse
- Strain or Cell Line
- Tg(Gnrh1-cre)1Dlc/J
- Source
- Jackson Labs
- Stock Number
- JAX: 021207
- Age at Injection
- 8 weeks
- Time After Injection
- 3 weeks
- Detection Method
- Immunohistochemistry, Direct fluorescence
- Assay & Results
- GnRH neurons in the median preoptic nucleus (MnPO).
- Target Cell Transduction
- Yes. We saw retrograde expression in expected regions after confirming endogenous fluorescence with immunohistochemistry.
- Functional Testing
- Optogenetic manipulations were not performed due to the sparseness of the labeled population.
- Other Information
- We did not see any toxicity