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pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA in Mouse (AAV Retrograde)

Addgene #20298-AAVrg

Data Submitted By
J. Elliott Robinson and Viviana Gradinaru
Submission Date
June 25, 2019
Publication Date
June 25, 2019 (modified May 16, 2023)
Not Provided
Caption: Left panel: Immunohistochemical labeling of Cre-dependent GFP expression via retrograde transgene delivery. Channel: 488 nm. Right panel: Immunohistochemical labeling of GnRH. Channel: 647 nm.
Image attribution: Gerry Coughlin

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vector Used
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

Virus & Injection


Virus Name
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
AAV Retrograde
Catalog Number
Cargo Type
Injection Titer / Dose
1.00E+13 GC/ml
Injection Volume
200 nl bilaterally
Injection Rate
20 nL/min
Injector Material
Not Provided
Injection Site / Route
Median eminence, NeuroNames: 402
Additional Details
GnRH neurons in the median eminence.
View in Addgene Catalog

Other Details

Strain or Cell Line
Jackson Labs
Stock Number
JAX: 021207
Age at Injection
8 weeks
Time After Injection
3 weeks
Detection Method
Immunohistochemistry, Direct fluorescence
Assay & Results
GnRH neurons in the median preoptic nucleus (MnPO).
Target Cell Transduction
Yes. We saw retrograde expression in expected regions after confirming endogenous fluorescence with immunohistochemistry.
Functional Testing
Optogenetic manipulations were not performed due to the sparseness of the labeled population.
Other Information
We did not see any toxicity