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pAAV-CAG-tdTomato (codon diversified) in Mouse (AAV Retrograde)

Addgene #59462-AAVrg

Data Submitted By
Nuria Vendrell Llopis and Jose Carmena
Submission Date
July 16, 2019
Publication Date
July 16, 2019 (modified May 16, 2023)
Abstract
Not Provided
Motor cortex 600u depth, red channel, 2p microscope, 20X objective
Caption: Motor cortex 600u depth, red channel, 2p microscope, 20X objective
Cortex 500u depth. Red channel, 2photon microscope, 20X objective, zoom=2,
Caption: Cortex 500u depth. Red channel, 2photon microscope, 20X objective, zoom=2,

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-CAG-tdTomato (codon diversified)
Addgene #59462-AAVrg

Virus & Injection

Virus

Virus Name
pAAV-CAG-tdTomato (codon diversified)
Serotype
AAV Retrograde
Source
Addgene
Catalog Number
59462-AAVrg
Promoter
CAG
Cargo Type
Control
Injection Titer / Dose
1.20E+13 GC/ml
Injection Volume
450 nl
Injection Rate
50 μl/min
Injector Material
Not Provided
Injection Site / Route
Cerebrospinal fluid (CSF): Intracerebroventricular (ICV); Hindbrain: Pons,NeuroNames ID: 547
Additional Details
I injected in the pons (AP: -4.26, ML: 0.6, DV: 4.6.) and looked for expression in the motor cortex.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
B6;DBA-Tg(tetO-GCaMP6s)2Niell/J
Source
Jackson Labs
Stock Number
JAX: 024742
Age at Injection
p45
Time After Injection
3 weeks
Detection Method
Direct fluorescence
Assay & Results
PT neurons in the motor cortex. I injected in the pons and looked for expression in the motor cortex. Not all the animals had somas in the field of view, but I could see axons in most of them.
Target Cell Transduction
PT neurons in the motor cortex.
Tips & Comments
It requires multiple animals to end up with an animal with sufficient expression. 60% of success rate that could improve with multiple injections instead of only one.