Skip to main content

pAAV-hSyn-dLight1.2 in Mouse (AAV5)

Addgene #111068-AAV5

Data Submitted By
Henry Hallock and Keri Martinowich
Submission Date
June 19, 2019
Publication Date
June 19, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Inscopix one-photon miniscope.
Image attribution: Henry Hallock

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-hSyn-dLight1.2
Addgene #111068-AAV5

Virus & Injection

Virus

Virus Name
pAAV-hSyn-dLight1.2
Serotype
AAV5
Source
Addgene
Catalog Number
111068-AAV5
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
8.30E+12 GC/ml
Injection Volume
two 400 nl injections
Injection Rate
200 nl/min
Injector Material
Not Provided
Injection Site / Route
Prelimbic area, NeuroNames ID: 1078
Additional Details
Targeted prelimbic sub-region of medial prefrontal cortex.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Jackson Labs
Stock Number
JAX: 000664
Age at Injection
3 months
Time After Injection
8 weeks
Detection Method
in vivo endoscopic imaging
Assay & Results
In vivo endoscopic imaging. Fluorescence was low with one-photon imaging, and couldn't make out individual ROIs, but can be used for bulk imaging.
Functional Testing
Tool did work - amphetamine (3 mg/kg) was used to increase dopamine levels in cortex.
Other Information
No toxicity - imaged with an Inscopix miniature head-mounted microscope and an endoscopic lens.