pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 in Rat (AAV9)
Addgene #100842-AAV9
- Data Submitted By
- Erin Glennon and Robert Froemke
- Submission Date
- July 09, 2019
- Publication Date
- July 09, 2019 (modified May 16, 2023)
- Abstract
- Not provided
Image attribution: Jasmin Multani
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
Addgene #100842-AAV9
Virus & Injection
Virus
- Virus Name
- pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 100842-AAV9
- Promoter
- CAG
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 5.20E+13 GC/mL
- Injection Volume
- two 1 μL injections
- Injection Rate
- 75 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Locus ceruleus, NeuroNames ID: 583
- Additional Details
- Noradrenergic cells of the locus coeruleus.
Other Details
- Species
- Rat
- Strain or Cell Line
- LE-Tg(TH-Cre)3.1Deis
- Source
- Rat Resource & Research Center
- Stock Number
- 659
- Age at Injection
- 3-4 months
- Time After Injection
- 1-8 weeks
- Detection Method
- Immunohistochemistry, fiber photometry
- Assay & Results
- Very limited expression in noradrenergic cells of locus coeruleus. Tested 1 week to 8 weeks after injection.
- Functional Testing
- Weak/unreliable signal on fiber photometry.
- Other Information
- Yes - I experienced toxicity in the sense that several animals developed seizures at the higher titers (undiluted or diluted 1:5), but I would get no GCaMP signal at lower titers.
- Tips & Comments
- I tested multiple capsids and promoters, and have found that AAV5-CAG works best.