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pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 in Mouse (AAV5)

Addgene #100845-AAV5

Data Submitted By
Francis Lee and Heidi Meyer
Submission Date
July 10, 2019
Publication Date
July 10, 2019 (modified May 16, 2023)
Not Provided
Caption: This image was taken at 10x and is the same brain slice as the previous image (provided to show spread of expression with 190 nl volume).
Caption: Image was taken at 10x magnification using a Nikon eclipse fluorescent microscope. Green cells indicate the presence of the Green Fluorescent Protein variant in the GCaMP molecule.

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vector Used

Virus & Injection


Virus Name
Catalog Number
Cargo Type
Injection Titer / Dose
1.10E+13 GC/mL
Injection Volume
190 nL
Injection Rate
50 nL/min
Injector Material
Not Provided
Injection Site / Route
Prelimbic area, NeuroNames ID: 1078
Additional Details
Prelimbic subregion of the prefrontal cortex. Targeting parvalbumin positive interneurons.
View in Addgene Catalog

Other Details

Strain or Cell Line
B6.129P2-Pvalbtm1(cre)Arbr/J obtained from Jackson Labs. Also known as B6 PVcre.
Jackson Labs
Stock Number
Jax: 017320
Age at Injection
postnatal day 21-23
Time After Injection
2 weeks
Detection Method
Direct fluorescence
Assay & Results
Expression was largely localized to the prelimbic cortex, with some expression also observed in the medial orbitofrontal cortex and infralimbic cortex. Expression was limited to neurons expressing parvalbumin.
Functional Testing
The tool operated as expected. A 460 nm light was used to excite the GCaMP, and emitted fluorescence was recorded (fiber photometry).
Other Information
No toxicity or inflammation