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pAAV-hSyn-hM3D(Gq)-mCherry in Rat (AAV2)

Addgene #50474-AAV2

Data Submitted By
Anthony A Grace and David Bortz
Submission Date
June 19, 2019
Publication Date
June 19, 2019 (modified May 16, 2023)
Not Reported
Caption: 10x image of expressing cell bodies in medial septum. Red is endogeous mCherry reporter. Green is mouse mCherry primary (1:8000, Abcam-ab125096) and goat anti-mouse secondary stain (alexa 488, 1:500; Abcam-ab150113). Yellow is co-stain overlay. Blue is Dapi.
Image attribution: David Bortz

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vector Used

Virus & Injection


Virus Name
Catalog Number
Cargo Type
Injection Titer / Dose
3.00E+12 GC/ml
Injection Volume
8 μL- 1 injection
Injection Rate
100 nl/min
Injector Material
Not Provided
Injection Site / Route
Medial septal nucleus NeuroNames ID : 262
Additional Details
I was trying to hit the anterior-posterior extent of the medial septum without getting into lateral septum or other parts of the basal forebrain, Medial septum.
View in Addgene Catalog

Other Details

Strain or Cell Line
Sprague Dawley, male from Envigo
Not provided
Age at Injection
Young adult (weight 300-325 grams)
Time After Injection
8 weeks for cell body expression, 12 weeks for terminal expression.
Detection Method
Assay & Results
Good expression on cell bodies within the medial septum as well as terminals in terminating regions. Cell type unknown.
Other Information
No toxicity detected
Tips & Comments
The one tip I would offer would be to leave the syringe at the place of infusion for several minutes following the infusion, and then raise it very slowly. This procedure has been effective for us in minimizing "dragging" the virus up with the syringe and out of the region of interest