pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 in Mouse (AAV1)
Addgene #100848-AAV1
- Data Submitted By
- Ruxandra Barzan and Dirk Jancke
- Submission Date
- July 03, 2020
- Publication Date
- July 03, 2020 (modified May 16, 2023)
- Abstract
- Not provided
Image attribution: Zohre Azimi
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40
Addgene #100848-AAV1
Virus & Injection
Virus
- Virus Name
- pAAV.Syn.NES.jRCaMP1a.WPRE.SV40
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 100848-AAV1
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- Two 0.5 μl injections
- Injection Rate
- 0.02 μl/min
- Injector Material
- Not provided
- Injection Site / Route
- Visual cortex, NeuroNames ID: 2102
- Additional Details
- Injections at two different sites within the primary visual cortex, each in 3 steps at depths between 600 and 200 μm below the brain surface. Break for 10 min between injection steps; Primary visual cortex
Other Details
- Species
- Mouse
- Strain or Cell Line
- mouse C57BL/6J, ePetCre
- Source
- Not provided
- Age at Injection
- 2 months
- Time After Injection
- 2 weeks
- Detection Method
- Direct fluorescence, wide-field calcium imaging
- Assay & Results
- Not Provided
- Functional Testing
- Good visually evoked calcium responses (see Azimi et al. , 2020; doi: 10. 7554/eLife. 53552).
- Other Information
- No toxicity or inflammation seen.