CN1851 Characterization
Addgene #164450
- Data Submitted By
- Boaz Levi , Bosiljka Tasic and Jonathan Ting
- Submission Date
- June 16, 2023
- Publication Date
- June 27, 2023 (modified June 27, 2023)
- DOI
- https://doi.org/10.57733/addgene.l0iuyl
- Funding
- NIA/NIMH RF1MH114126, UF1MH128339
- Abstract
- Forebrain GABAergic neurons are labeled in mouse brain by transduction with CN1851 AAV of the PHP.eB serotype. It was injected at a concentration of 1.0E+11 viral genomes per animal into one month-old Ai14 Cre recombinase reporter mice via the retro-orbital route. The animal was perfused and the brain fixed in 4% paraformaldehyde four weeks post injection. The fixed brain tissue was then sectioned, slide mounted, and imaged for native tdTomato fluorescence. CN1851 has been characterized and was demonstrated to label forebrain GABAergic neurons in Graybuck et. al. (Neuron) 2021. https://doi.org/10.1016/j.neuron.2021.03.011.
- Citation
-
CN1851 Characterization. Levi B, Tasic B, Ting J 2023. Addgene Report, https://doi.org/10.57733/addgene.l0iuyl
Image attribution: VGT Pipeline, Allen Institute for Brain Science
This report is made available under the Creative Commons Attribution 4.0 International License.
- Vector Used
-
CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA
Addgene #164450
Virus & Injection
Virus
- Virus Name
- CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA
- Serotype
- PHP.eB
- Source
- Addgene
- Catalog Number
- 164450
- Virus Lot Number
- v165478G
- Promoter
- Other
- Other Promoter
- hI56i
- Cargo Type
- Recombinase
- Injection Titer / Dose
- 1.0 x 10e11 vg/animal
- Injection Volume
- 90 µl
- Injection Rate
- N/A
- Injector Material
- 31g insulin syringe
- Tip Diameter
- 31
- Coatings
- N/A
- Injection Time
- N/A
- Injection Site / Route
- Retro-orbital injection
- Additional Details
- AAV-PHP.eB/CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA was injected at a concentration of 1.0 x 10e11 viral genomes per animal into one month-old Ai14 Cre recombinase reporter mice via the retro-orbital route.
Other Details
- Species
- Mouse
- Strain or Cell Line
- Ai14(RCL-tdT): B6.CgGt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
- Source
- Jackson Laboratory
- Stock Number
- 007914
- Age at Injection
- 1 month
- Sex
- Male
- Time After Injection
- 4 Weeks
- Detection Method
- Native fluorescence
- Assay & Results
- The animal was perfused and the brain fixed with 4% paraformaldehyde four weeks post injection. The fixed brain tissue was then sectioned, slide mounted, and imaged for native tdTomato fluorescence on a Leica Aperio Versa slide scanner.
- Target Cell Transduction
- N/A
- Functional Testing
- iCre was functional as expected.