AAV-hSyn1-GCaMP6s-P2A-nls-dTomato in Human (ex: human brain slices) (AAV1)
Addgene #51084-AAV1
- Data Submitted By
- Brady Maher and Srinidhi Rao Sripathy
- Submission Date
- June 27, 2019
- Publication Date
- June 27, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: Srinidhi Rao
Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.
- Vector Used
-
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato
#51084-AAV1
Virus & Injection
Virus
- Virus Name
- AAV-hSyn1-GCaMP6s-P2A-nls-dTomato
- Serotype
- AAV1
- Catalog Number
- 51084-AAV1
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- 1μl
- Injection Rate
- N/A
- Injector Material
- N/A
- Injection Site / Route
- Culture dish
- Additional Details
- Neurons cultured in a dish.
Other Details
- Species
- Human
- Strain or Cell Line
- Not Provided
- Source
- N/A
- Age at Injection
- 10 week old neurons
- Time After Injection
- 3 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- 1uL of virus for every 24 well provides sufficient infection.
- Functional Testing
- Yes, we use to check innate calcium signals
- Other Information
- A small level of cell death. But very small.