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AAV-hSyn1-GCaMP6s-P2A-nls-dTomato in Human (ex: human brain slices) (AAV1)

Addgene #51084-AAV1

Data Submitted By
Brady Maher and Srinidhi Rao Sripathy
Submission Date
June 27, 2019
Publication Date
June 27, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Green channel is cells expressing GCamp
Image attribution: Srinidhi Rao

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vector Used
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato
#51084-AAV1

Virus & Injection

Virus

Virus Name
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato
Serotype
AAV1
Catalog Number
51084-AAV1
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
1.00E+13 GC/ml
Injection Volume
1μl
Injection Rate
N/A
Injector Material
N/A
Injection Site / Route
Culture dish
Additional Details
Neurons cultured in a dish.
View in Addgene Catalog

Other Details

Species
Human
Strain or Cell Line
Not Provided
Source
N/A
Age at Injection
10 week old neurons
Time After Injection
3 weeks
Detection Method
Direct fluorescence
Assay & Results
1uL of virus for every 24 well provides sufficient infection.
Functional Testing
Yes, we use to check innate calcium signals
Other Information
A small level of cell death. But very small.