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CN1839 Characterization

Addgene #163509

Data Submitted By
Boaz Levi , Bosijka Tasic and Jonathan Ting
Submission Date
June 16, 2023
Publication Date
June 27, 2023 (modified June 27, 2023)
DOI
https://doi.org/10.57733/addgene.dibjsm
Funding
NIA/NIMH RF1MH114126, UF1MH128339
Abstract
Neuronal nuclei are labeled throughout the mouse brain by transduction with CN1839 AAV of the PHP.eB serotype. It was injected at a concentration of 5.0E+11 viral genomes per animal into one month-old C57BL/6J mice via the retro-orbital route. The animal was perfused and the brain fixed in 4% paraformaldehyde four weeks post injection. The fixed brain tissue was then sectioned, slide mounted, and imaged for native SYFP2 fluorescence. CN1839 has been characterized and shown to label neurons throughout the mouse brain in Mich et. al. (Cell Reports) 2021. https://doi.org/10.1016/j.celrep.2021.108754.
Citation
CN1839 Characterization. Levi B, Tasic B, Ting J 2023. Addgene Report, https://doi.org/10.57733/addgene.dibjsm
Caption: A sagittal section of a whole mouse brain is shown on the left, and a portion of the cortex is shown on the right (including VISp and PFLp, cortical areas marked by yellow box). Forebrain GABAergic cells are labeled in mouse brain by transduction with AAV-PHP.eB/CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA. CN1839 has been extensively characterized and shown to label forebrain GABAergic cells in Mich et. al. (Cell Reports) 2021. https://doi.org/10.1016/j.celrep.2021.108754.
Image attribution: VGT Pipeline, Allen Institute for Brain Science

This report is made available under the Creative Commons Attribution 4.0 International License.

Vector Used
CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA
Addgene #163509

Virus & Injection

Virus

Virus Name
CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA
Serotype
PHP.eB
Source
Addgene
Catalog Number
163509
Virus Lot Number
v164542G
Promoter
Synapsin
Cargo Type
Control
Injection Titer / Dose
5.0 x 10e11
Injection Volume
90 ┬Ál
Injection Rate
N/A
Injector Material
31g insulin syringe
Tip Diameter
31
Coatings
N/A
Injection Time
N/A
Injection Site / Route
Retro-orbital injection
Additional Details
AAV-PHP.eB/CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA was injected at a concentration of 5.0 x 10e11 viral genomes per animal into month-old C57BL/6J mice via the retro-orbital route.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57BL/6J
Source
Jackson Laboratory
Stock Number
000664
Age at Injection
1 month
Sex
Male
Time After Injection
4 Weeks
Detection Method
Native Fluorescence
Assay & Results
The animal was perfused and the brain fixed with 4% paraformaldehyde four weeks post injection. The fixed brain tissue was then sectioned, slide mounted, and imaged for native SYFP2 fluorescence on a Leica Aperio Versa slide scanner.
Target Cell Transduction
N/A
Functional Testing
N/A