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pENN.AAV.hSyn.Cre.WPRE.hGH in Rat (AAV Retrograde)

Addgene #105553-AAVrg

Data Submitted By
Todd Nentwig and L. Judson Chandler
Submission Date
July 31, 2019
Publication Date
July 31, 2019 (modified May 16, 2023)
Abstract
Not Provided
Prelimbic cortex neurons labeled by mouse anti-Cre antibody following injection of retrograde Cre virus into medio dorsal thalamic region
Caption: Prelimbic cortex neurons labeled by mouse anti-Cre antibody following injection of retrograde Cre virus into medio dorsal thalamic region

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pENN.AAV.hSyn.Cre.WPRE.hGH
Addgene #105553-AAVrg

Virus & Injection

Virus

Virus Name
pENN.AAV.hSyn.Cre.WPRE.hGH
Serotype
AAV Retrograde
Source
Addgene
Catalog Number
105553-AAVrg
Promoter
Synapsin
Cargo Type
Recombinase
Injection Titer / Dose
1.20E+13 GC/ml
Injection Volume
200 nl
Injection Rate
60 nl/min
Injector Material
Not Provided
Injection Site / Route
Habenula, NeuroNames ID: 294
View in Addgene Catalog

Other Details

Species
Rat
Strain or Cell Line
Long-Evans
Source
Not provided
Age at Injection
~100 days old
Time After Injection
8-12 weeks
Detection Method
Immunohistochemistry
Assay & Results
Targeted neurons (hSyn promoter) in the Lateral Habenula, and retrogradely labeled brain areas that project to the Lateral Habenula (eg, Lateral Hypothalamus).
Target Cell Transduction
The virus operated as expected. Retrograde labeling was detected via immunohistochemistry in region that project to the brain region targeted by the virus injection.
Other Information
No oddities were observed in the studies using this virus