pAAV-hSyn-hM3D(Gq)-mCherry in Rat (AAV8)
Addgene #50474-AAV8
- Data Submitted By
- Todd Nentwig and L. Judson Chandler
- Submission Date
- December 19, 2020
- Publication Date
- December 19, 2020 (modified May 16, 2023)
- Abstract
- Not provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-hSyn-hM3D(Gq)-mCherry
Addgene #50474-AAV8
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-hM3D(Gq)-mCherry
- Serotype
- AAV8
- Source
- Addgene
- Catalog Number
- 50474-AAV8
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 2.00E+12 GC/ml
- Injection Volume
- 450nl
- Injection Rate
- 1nl/sec
- Injector Material
- Not provided
- Injection Site / Route
- Reticular nucleus of the thalamus NeuroNames ID: 366
- Additional Details
- Forebrain: Basal Ganglia: Globus pallidus internal or entopeduncular nucleus
Other Details
- Species
- Rat
- Strain or Cell Line
- Long-Evans
- Age at Injection
- ~100 days old
- Time After Injection
- 4 weeks
- Detection Method
- Immunohistochemistry
- Functional Testing
- Neurons expressing the excitatory DREADD exhibited robust induction of cFos following systemic injection of DREADD ligand, Deschloroclozapine