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pAAV-hsyn-Jaws-KGC-GFP-ER2 in Rhesus macaque (AAV5)

Addgene #65014-AAV5

Data Submitted By
Adriana Galvan
Submission Date
June 27, 2019
Publication Date
June 27, 2019 (modified May 16, 2023)
Not Provided
Caption: Expression of Jaws on GPi neurons. AAV5-hSyn-Jaws-KGC-GFP-ER2 was injected in the internal globus pallidus (GPi) of a rhesus macaque. The area in the rectangle is shown at higher magnification in the inset. Jaws expression was revealed with antibodies against GFP and immunoperoxidase. GPe, external globus pallidus, OT, optic tract. ((Galvan et al., unpublished data)­

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vector Used

Virus & Injection


Virus Name
Catalog Number
Cargo Type
Injection Titer / Dose
3.60E+13 GC/mL
Injection Volume
18 μL
Injection Rate
100 nL/min, increasing to 200 nL/min
Injector Material
Not Provided
Injection Site / Route
Lateral globus pallidus, NeuroNames ID: 232
View in Addgene Catalog

Other Details

Non-Human Primate
Strain or Cell Line
Rhesus macaque
Not provided
Age at Injection
5 years (subject 1); and 5.5 years (subject 2)
Time After Injection
6 to 20 weeks
Detection Method
Immunohistochemistry, Optogenetics
Assay & Results
Trying to target all neurons in Globus pallidus external (GPe ). Jaws expression was revealed with antibodies against GFP and immunoperoxidase. Neurons at injection site (GPe neurons), neurons in neighboring regions (GPi, likely due to spread of virus), and expression of GFP along axons and terminals originating form GPe cells (anterograde transport).
Functional Testing
The opsin Jaws silenced neurons in GPe when activated by light.
Tips & Comments
Inject a smaller volume to keep the expression confined to the region of interest.