pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 in Mouse (AAV9)
Addgene #108912-AAV9

DAPI (magenta) and mRuby3 (red) expression in thalamic reticular nucleus. Magnification 40x.

Caption: DAPI (magenta) and mRuby3 (red) expression in thalamic reticular nucleus. Magnification 40x.

Image attribution: Atehsa Sahagun, Kevin Bender

Virus & Injection

Primary Virus
Promoter
CAG
Injected Titer
1.00E+13 GC/ml
Injected Volume
two 200 nl injections
Injection Rate
100 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Thalamus
Injection Site Comments
Thalamic reticular nucleus - GABAergic neurons
View in Addgene Catalog

Subject

Species / Strain
Mouse PV-cre heterozygote
Age at Injection
P28

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence, Whole-cell patch clamp
Expression Site
TRN neurons
Genetic Tool Outcome
ChroME, 1040 nm light used to drive spiking
Other Information
At 9 weeks* of expression, mRuby was widely diffuse, not nuclear. *Opportunity provided by covid-19