pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 in Mouse (AAV9)
Addgene #108912-AAV9
Virus & Injection
- Primary Virus
- Promoter
- CAG
- Injected Titer
- 1.00E+13 GC/ml
- Injected Volume
- two 200 nl injections
- Injection Rate
- 100 nl/min
- Injection Site
- Brain parenchyma
- Brain Region
- Forebrain: Thalamus
- Injection Site Comments
- Thalamic reticular nucleus - GABAergic neurons
Subject
- Species / Strain
- Mouse PV-cre heterozygote
- Age at Injection
- P28
Data Collection
- Time After Injection
- 3 weeks
- Detection Method
- Direct fluorescence, Whole-cell patch clamp
- Expression Site
- TRN neurons
- Genetic Tool Outcome
- ChroME, 1040 nm light used to drive spiking
- Other Information
- At 9 weeks* of expression, mRuby was widely diffuse, not nuclear. *Opportunity provided by covid-19