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pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 in Mouse (AAV9)
Addgene #108912-AAV9

DAPI (magenta) and mRuby3 (red) expression in thalamic reticular nucleus. Magnification 40x. — Caption: DAPI (magenta) and mRuby3 (red) expression in thalamic reticular nucleus. Magnification 40x.

Image attribution: Atehsa Sahagun, Kevin Bender

Data Submitted By

Julie Haas

Submission date: July 17, 2020

Any further use of this image requires the permission of the lab.

This scientist responded to a specific request for user data and was offered compensation for time and input.

Vectors Used

pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 #108912-AAV9 deposited by Hillel Adesnik

Virus & Injection

Primary Virus: pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 (AAV9)
Promoter: CAG
Injected Titer: 1.00E+13 GC/ml
Injected Volume: two 200 nl injections
Injection Rate: 100 nl/min
Injection Site: Brain parenchyma
Brain Region: Forebrain: Thalamus
Injection Site Comments: Thalamic reticular nucleus - GABAergic neurons

View in Addgene Catalog

Subject

Species / Strain: Mouse PV-cre heterozygote
Age at Injection: P28

Data Collection

Time After Injection: 3 weeks
Detection Method: Direct fluorescence, Whole-cell patch clamp
Expression Site: TRN neurons
Genetic Tool Outcome: ChroME, 1040 nm light used to drive spiking
Other Information: At 9 weeks* of expression, mRuby was widely diffuse, not nuclear. *Opportunity provided by covid-19