pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 in Mouse (AAV2)
Addgene #105545-AAV2

LDT (laterodorsal tegmental nucleus) neurons expressing retro-AAV-eGFP-cre. Cre: GFP (green); DAPI (blue). A Zeiss AxioImager M2 upright widefield fluorescence/differential interference contrast microscope (5x objective) was used to obtain the image.

Caption: LDT (laterodorsal tegmental nucleus) neurons expressing retro-AAV-eGFP-cre. Cre: GFP (green); DAPI (blue). A Zeiss AxioImager M2 upright widefield fluorescence/differential interference contrast microscope (5x objective) was used to obtain the image.

Virus & Injection

Primary Virus
Promoter
CMV
Injected Titer
1.00E+12 GC/ml
Injected Volume
300 nl
Injection Rate
150 nl/min
Injection Site
Brain parenchyma
Brain Region
Midbrain: Tegmentum
Injection Site Comments
Injection of the virus to VTA to map the inputs from LDTg
View in Addgene Catalog

Subject

Species / Strain
Mouse C57BL/6J6J
Age at Injection
7 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
Excitatory neurons in the brain stem area
Genetic Tool Outcome
It worked as expected
Other Information
No toxicity or inflammation were observed via anatomic imaging within 3 weeks after virus infusion
Tips & Comments
The toxicity should be evaluated using in vivo recording in future. But so far, this virus works really well