AAV pCAG-FLEX-tdTomato-WPRE in Mouse (AAV1)
Addgene #51503-AAV1

The image was acquired from mouse brain 4 weeks after photothrombotic ischemia in the area of the motor cortex. Virus is shown in red We acquired the image with a Olympus BX43 and a Filter for Cy3 with a 4x Objective.

Caption: The image was acquired from mouse brain 4 weeks after photothrombotic ischemia in the area of the motor cortex. Virus is shown in red We acquired the image with a Olympus BX43 and a Filter for Cy3 with a 4x Objective.

Virus & Injection

Primary Virus
Promoter
CAG
Injected Volume
1000 nl
Injection Rate
0.1nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Injected into the layer 5 of the primary motor cortex, targeting the pyramidal tract, descending from the motor cortex. Coordinates: M/L: 1. 75 A/P: 1. 10 D/V: -0. 8
View in Addgene Catalog

Subject

Species / Strain
Mouse B6.129S2-Emx1tm1(cre)Krj/J
Stock Number
Jax mouse stock No: 005628
Age at Injection
8 weeks

Data Collection

Time After Injection
8 weeks
Detection Method
Immunohistochemistry
Expression Site
Pyramidal neurons of the corticospinal tract
Other Information
Viral prep was obtained from the University of Pennsylvania Vector Core (Penn Vector Core)