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AAV pCAG-FLEX-tdTomato-WPRE in Mouse (AAV1)

Addgene #51503-AAV1

Data Submitted By
Frederique Wieters and Markus Aswendt
Submission Date
June 17, 2020
Publication Date
June 17, 2020 (modified May 16, 2023)
Abstract
Not provided
The image was acquired from mouse brain 4 weeks after photothrombotic ischemia in the area of the motor cortex. Virus is shown in red We acquired the image with a Olympus BX43 and a Filter for Cy3 with a 4x Objective.
Caption: The image was acquired from mouse brain 4 weeks after photothrombotic ischemia in the area of the motor cortex. Virus is shown in red We acquired the image with a Olympus BX43 and a Filter for Cy3 with a 4x Objective.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
AAV pCAG-FLEX-tdTomato-WPRE
Addgene #51503-AAV1

Virus & Injection

Virus

Virus Name
AAV pCAG-FLEX-tdTomato-WPRE
Serotype
AAV1
Source
Addgene
Catalog Number
51503-AAV1
Promoter
CAG
Cargo Type
Control
Injection Titer / Dose
Not provided
Injection Volume
1000 nl
Injection Rate
0.1nl/min
Injector Material
Not provided
Injection Site / Route
Primary motor cortex, NeuroNames ID: 1911
Additional Details
Injected into the layer 5 of the primary motor cortex, targeting the pyramidal tract, descending from the motor cortex. Coordinates: M/L: 1. 75 A/P: 1. 10 D/V: -0. 8; Forebrain: Cerebral cortex (neocortex); Pyramidal neurons of the corticospinal tract
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
B6.129S2-Emx1tm1(cre)Krj/J
Stock Number
Jax mouse stock No: 005628
Age at Injection
8 weeks
Time After Injection
8 weeks
Detection Method
Immunohistochemistry
Other Information
Viral prep was obtained from the University of Pennsylvania Vector Core (Penn Vector Core)