pAAV.GfaABC1D.PI.Lck-GFP.SV40 in Rat (AAV5)
Addgene #105598-AAV5

Rat Lateral Habenula; 10x mag. Astrocytes labeled using GfaABC1D.PI.Lck-GFP virus (green). Synapsin and Glutamate Transporter 1 (GLT1) are labeled with red and cyan staining, respectively.

Caption: Rat Lateral Habenula; 10x mag. Astrocytes labeled using GfaABC1D.PI.Lck-GFP virus (green). Synapsin and Glutamate Transporter 1 (GLT1) are labeled with red and cyan staining, respectively.

Image attribution: Anna Kruyer

Virus & Injection

Primary Virus
Promoter
GFAP
Injected Titer
7.00E+12 GC/ml
Injected Volume
200 nl
Injection Rate
1 nl/sec
Injection Site
Brain parenchyma
Brain Region
Lateral habenula
View in Addgene Catalog

Subject

Species / Strain
Rat Sprague-Dawley
Age at Injection
~150 days

Data Collection

Time After Injection
4 weeks
Detection Method
Direct fluorescence
Expression Site
Lateral Habenula Astrocytes
Tips & Comments
Direct fluorescence was sufficient to visualize site/spread of virus expression but immuno-amplification is likely necessary if performing high magnification, quantitative analysis of astrocyte-neuron interactions