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pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 in Mouse (AAV5)

Addgene #100842-AAV5

Data Submitted By: Kengo Inada and Kazunari Miyamichi
Submission Date: August 11, 2019
Publication Date: August 11, 2019 (modified May 16, 2023)
Abstract: Not Provided

GCaMP expressing neurons are visualized by anti-GFP staining (green). Coronal section. Blue, DAPI, 3V, third ventricle. Scale bar, 100 um. — Caption: GCaMP expressing neurons are visualized by anti-GFP staining (green). Coronal section. Blue, DAPI, 3V, third ventricle. Scale bar, 100 um.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used: pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
Addgene #100842-AAV5

Virus & Injection

Virus

Virus Name: pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
Serotype: AAV5
Source: Addgene
Catalog Number: 100842-AAV5
Promoter: CAG
Cargo Type: Biosensor
Injection Titer / Dose: 3.20E+13 GC/mL
Injection Volume: 400 nL
Injection Rate: 50 nL/min
Injector Material: Not Provided
Injection Site / Route: Paraventricular nucleus of the hypothalamus, NeuroNames ID: 387

View in Addgene Catalog

Other Details

Species: Mouse
Strain or Cell Line: C57/BL6J
Source: Jackson Labs
Stock Number: 024234
Age at Injection: 6 weeks old
Time After Injection: 2 weeks
Detection Method: Immunohistochemistry, Direct fluorescence
Assay & Results: Oxytocinergic neurons in paraventricular hypothalamic nucleus. GCaMP expressing neurons are visualized by anti-GFP staining (green).
Functional Testing: Yes