pAAV.CAG.GCaMP6s.WPRE.SV40 in Mouse (AAV5)
Addgene #100844-AAV5
- Data Submitted By
- Dan Levy
- Submission Date
- July 29, 2019
- Publication Date
- July 29, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV.CAG.GCaMP6s.WPRE.SV40
Addgene #100844-AAV5
Virus & Injection
Virus
- Virus Name
- pAAV.CAG.GCaMP6s.WPRE.SV40
- Serotype
- AAV5
- Source
- Addgene
- Catalog Number
- 100844-AAV5
- Promoter
- CAG
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 5.00E+12 GC/ml
- Injection Volume
- Two 2 μl injections
- Injection Rate
- 200 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Trigeminal ganglion, NeuroNames ID: 1402
- Additional Details
- Targeting sensory neurons. Trigeminal ganglion and cranial meninges
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/Bl6J
- Age at Injection
- Time After Injection
- 8 weeks
- Detection Method
- Immunohistochemistry, two-photon
- Tips & Comments
- Tested AAV1 with the same cargo with slightly better success rate (75% for AAV1 vs 25-45% with AAV5)