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pAAV-GFAP-hM4D(Gi)-mCherry in Rat (AAV5)

Addgene #50479-AAV5

Data Submitted By: Zafar Iqbal and Ying Li
Submission Date: July 28, 2019
Publication Date: July 28, 2019 (modified May 16, 2023)
Abstract: Not Provided

hm4Di (red) colabel with astrocytes (Green) and DAPi (blue) — Caption: hm4Di (red) colabel with astrocytes (Green) and DAPi (blue)

Any further use of this image requires permission from the lab who submitted the data.

Vector Used: pAAV-GFAP-hM4D(Gi)-mCherry
Addgene #50479-AAV5

Virus & Injection

Virus

Virus Name: pAAV-GFAP-hM4D(Gi)-mCherry
Serotype: AAV5
Source: Addgene
Catalog Number: 50479-AAV5
Promoter: GFAP
Cargo Type: Chemogenetics
Injection Titer / Dose: 2.50E+12 GC/ml
Injection Volume: 1 μl
Injection Rate: 100 nl/min
Injector Material: Not Provided
Injection Site / Route: Hippocampus NeuroNames ID : 3157
Additional Details: Astrocytes; Forebrain: Hippocampus; HPC astrocytes

View in Addgene Catalog

Other Details

Species: Rat
Strain or Cell Line: Sprague Dawley
Source: Not provided
Age at Injection: 250 to 300grams
Time After Injection: 2 weeks
Detection Method: Immunohistochemistry
Assay & Results: Not Provided
Functional Testing: CNO was used to activate hm4Di