pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 in Mouse (AAV1)
Addgene #100833-AAV1
- Data Submitted By
- Qijun Tang and Ali Guler
- Submission Date
- July 25, 2019
- Publication Date
- July 25, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV.Syn.Flex.GCaMP6f.WPRE.SV40
Addgene #100833-AAV1
Virus & Injection
Virus
- Virus Name
- pAAV.Syn.Flex.GCaMP6f.WPRE.SV40
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 100833-AAV1
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 4.00E+12 GC/ml
- Injection Volume
- 500 nl
- Injection Rate
- 100 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Nucleus accumbens NeuroNames ID : 277 caudate
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J with Drd1-Cre
- Source
- Not provided
- Age at Injection
- 10 weeks
- Time After Injection
- 1 week
- Detection Method
- Direct fluorescence
- Assay & Results
- Nucleus accumbens, Drd1 neurons are targeted. Only in the targeted region. Very good expression.
- Functional Testing
- Yes
- Other Information
- Cited in: Deletion of GAD67 in dopamine receptor-1 expressing cells causes specific motor deficits (https://doi.org/10.1002/dvg.20405)