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pAAV-hSyn-hM4D(Gi)-mCherry in Mouse (AAV2)

Addgene #50475-AAV2

Data Submitted By
Ting Feng and Simon Pieraut
Submission Date
July 25, 2019
Publication Date
July 25, 2019 (modified May 16, 2023)
Abstract
Not Provided
Coronal mouse brain section of the MEC layer 2 long range projections in dentate gyrus labeling with DREADD receptor hM3D or hM4D was taken by Leica confocal microscope under 20X. Red channel represents mCherry, and blue color is DAPI.
Caption: Coronal mouse brain section of the MEC layer 2 long range projections in dentate gyrus labeling with DREADD receptor hM3D or hM4D was taken by Leica confocal microscope under 20X. Red channel represents mCherry, and blue color is DAPI.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-hSyn-hM4D(Gi)-mCherry
Addgene #50475-AAV2

Virus & Injection

Virus

Virus Name
pAAV-hSyn-hM4D(Gi)-mCherry
Serotype
AAV2
Source
Addgene
Catalog Number
50475-AAV2
Promoter
Synapsin
Cargo Type
Chemogenetics
Injection Titer / Dose
2.00E+12 GC/ml
Injection Volume
0.5 μl
Injection Rate
Not Provided
Injector Material
Not Provided
Injection Site / Route
Medial entorhial cortex NeuroNames ID : 1113
Additional Details
Medial entorhial cortex provides input to the dentate gyrus. Our goal is to target the perforate path fiber in dentate gyrus.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Not provided
Age at Injection
P1 to P3
Time After Injection
2 or 3 weeks
Detection Method
Direct fluorescence
Assay & Results
Not Provided
Functional Testing
We used CNO to active the hM3D and hM4d. We saw the c-fos expression was changed dramatically
Tips & Comments
The expression pattern looked the same for the excitatory DREADD (50474-AAV2)