AAV.CamKII.GCaMP6s.WPRE.SV40 in Mouse (AAV9)
Addgene #107790-AAV9
- Data Submitted By
- Michael Sokoletsky and Ilan Lampl
- Submission Date
- July 21, 2019
- Publication Date
- July 21, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
AAV.CamKII.GCaMP6s.WPRE.SV40
Addgene #107790-AAV9
Virus & Injection
Virus
- Virus Name
- AAV.CamKII.GCaMP6s.WPRE.SV40
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 107790-AAV9
- Promoter
- CaMKII
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 2.00E+11 GC/ml
- Injection Volume
- 500 nl
- Injection Rate
- 30 nl/min to prevent backflow
- Injector Material
- Not Provided
- Injection Site / Route
- Whisker sensory area (Barrel Cortex) NeuroNames ID : 2703
- Additional Details
- I was targeting all excitatory cells in the barrel cortex; Forebrain: Cerebral cortex (neocortex); Expression was local to the barrel cortex. Assumed excitatory cells, but we did not attempt to verify co-localization between the virus expression and CamKII as these experiments were largely used for testing
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Not provided
- Age at Injection
- 6-10 weeks
- Time After Injection
- 2 weeks
- Detection Method
- Immunohistochemistry, Direct fluorescence
- Assay & Results
- Expression was local to the barrel cortex. Assumed excitatory cells, but we did not attempt to verify co-localization between the virus expression and CamKII as these experiments were largely used for testing.
- Other Information
- Injection was at multiple sites (-300 um, -450 um, and -600 um) below the dura to prevent backflow but also spread the virus more equally