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pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV8)

Addgene #44361-AAV8

Data Submitted By
Avishek Adhikari
Submission Date
July 18, 2019
Publication Date
July 18, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Dreadd (hm3d-mcherry) in red (610 emission), in cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line, epifluorescence microscope, 4x objective

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Addgene #44361-AAV8

Virus & Injection

Virus

Virus Name
pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Serotype
AAV8
Source
Addgene
Catalog Number
44361-AAV8
Promoter
Synapsin
Cargo Type
Chemogenetics
Injection Titer / Dose
3.00E+12 GC/ml
Injection Volume
60 nl
Injection Rate
300 nl/min
Injector Material
Not Provided
Injection Site / Route
Hypothalamus NeuroNames ID : 375
Additional Details
Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line; Forebrain: Hypothalamus; Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Jackson Labs
Stock Number
JAX: 000664
Age at Injection
2 months
Time After Injection
8 weeks
Detection Method
Direct fluorescence
Assay & Results
Not Provided
Functional Testing
The DREADD receptor worked as expected upon treatment with systemic CNO injection