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AAV-FLEX-Arch-GFP in Mouse (AAV9)

Addgene #22222-AAV9

Data Submitted By: Avishek Adhikari
Submission Date: July 18, 2019
Publication Date: July 18, 2019 (modified May 16, 2023)
Abstract: Not Provided

cck+ cells in hypothalamus, arch-gfp (green) is 510 nm emission channel, 5x objective — Caption: cck+ cells in hypothalamus, arch-gfp (green) is 510 nm emission channel, 5x objective

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Vector Used: AAV-FLEX-Arch-GFP
Addgene #22222-AAV9

Virus & Injection

Virus

Virus Name: AAV-FLEX-Arch-GFP
Serotype: AAV9
Source: Addgene
Catalog Number: 22222-AAV9
Promoter: CAG
Cargo Type: Control
Injection Titer / Dose: 2.00E+12 GC/ml
Injection Volume: 40 nl
Injection Rate: 300 nL/min
Injector Material: Not Provided
Injection Site / Route: Hypothalamus NeuroNames ID : 375
Additional Details: Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line;

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Other Details

Species: Mouse
Strain or Cell Line: C57/BL6J
Source: Jackson Labs
Stock Number: JAX: 000664
Age at Injection: 2 months
Time After Injection: 8 weeks
Detection Method: Direct fluorescence
Assay & Results: Not Provided
Functional Testing: Green light was used to activate arch, and the behavior worked