pAAV-CAG-tdTomato (codon diversified) in Mouse (AAV Retrograde)
Addgene #59462-AAVrg
- Data Submitted By
- Nuria Vendrell Llopis and Jose Carmena
- Submission Date
- July 16, 2019
- Publication Date
- July 16, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-CAG-tdTomato (codon diversified)
Addgene #59462-AAVrg
Virus & Injection
Virus
- Virus Name
- pAAV-CAG-tdTomato (codon diversified)
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 59462-AAVrg
- Promoter
- CAG
- Cargo Type
- Control
- Injection Titer / Dose
- 1.20E+13 GC/ml
- Injection Volume
- 450 nl
- Injection Rate
- 50 μl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Motor cortex NeuroNames ID: 2332
- Additional Details
- Motor Cortex (contralateral of area of imaging).
Other Details
- Species
- Mouse
- Strain or Cell Line
- B6;DBA-Tg(tetO-GCaMP6s)2Niell/J
- Source
- Jackson Labs
- Stock Number
- JAX: 024742
- Age at Injection
- p54
- Time After Injection
- 3 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Nice expression in the contralateral as well as in the ipsilateral motor cortex.
- Target Cell Transduction
- Targeting IT neurons in the contralateral motor cortex.
- Other Information
- No toxicity no inflammation