pAAV.hSynapsin.SF-iGluSnFR.A184S in Mouse (AAV1)
Addgene #106174-AAV1
- Data Submitted By
- Veronica Diaz and Dayu Lin
- Submission Date
- July 16, 2019
- Publication Date
- July 16, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: null
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV.hSynapsin.SF-iGluSnFR.A184S
Addgene #106174-AAV1
Virus & Injection
Virus
- Virus Name
- pAAV.hSynapsin.SF-iGluSnFR.A184S
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 106174-AAV1
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- 50 nL
- Injection Rate
- 10 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Shell of the nucleus accumbens, NeuroNames ID: 1163
Other Details
- Species
- Mouse
- Strain or Cell Line
- wild type swiss webster
- Source
- Not provided
- Age at Injection
- 8 months
- Time After Injection
- 3 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Wild type, all cells. Subregion targeted: nucleus accumbens medial shell. Nucleus accumbens medial shell with minor spillover to ventral lateral septum (no cell specificity).
- Functional Testing
- Good signal detected using fiber photometry.
- Tips & Comments
- Tends to spread a bit, would inject less than GCamp, for example.