pAAV-Syn-ChrimsonR-tdT in Mouse (AAV9)
Addgene #59171-AAV9
- Data Submitted By
- Antonios Pouliopoulos and Elisa Konofagou
- Submission Date
- July 11, 2019
- Publication Date
- July 11, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
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- Vector Used
-
pAAV-Syn-ChrimsonR-tdT
Addgene #59171-AAV9
Virus & Injection
Virus
- Virus Name
- pAAV-Syn-ChrimsonR-tdT
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 59171-AAV9
- Promoter
- Synapsin
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 1.70E+12 GC/ml
- Injection Volume
- 100 μl
- Injection Rate
- N/A
- Injector Material
- N/A
- Injection Site / Route
- Intravenous, tail vein
- Additional Details
- Systemic injection through the tail vein. The blood-brain barrier was locally and non-invasively disrupted using focused ultrasound. We intended to transfect neurons within the cortex and hippocampus; Cortical and hippocampal neurons
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Not provided
- Age at Injection
- 3-6 months
- Time After Injection
- 3 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Not Provided
- Functional Testing
- It operated as expected. Red light (635nm) was used to activate the neural network. Activation was detected through cFos staining post-mortem
- Other Information
- No toxicity or inflammation due to the AAV expression was observed
- Tips & Comments
- PAAV-Syn-ChrimsonR-tdT (AAV9) employs the Syn promoter. Other promoters targeting more specific neuronal types would be welcome