pAAV-hSyn-DIO-mCherry in Mouse (AAV5)

Addgene #50459-AAV5

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Vectors Used: pAAV-hSyn-DIO-mCherry
Addgene #50459-AAV5; pENN.AAV.hSyn.Cre.WPRE.hGH
Addgene #105553-AAVrg

Virus Name: pAAV-hSyn-DIO-mCherry
Serotype: AAV5
Source: Addgene
Catalog Number: 50459-AAV5
Promoter: Synapsin
Cargo Type: Control
Injection Titer / Dose: 4.10E+12 GC/ml
Injection Volume: 600 nl
Injection Rate: 200 nl/min
Injector Material: Not Provided
Injection Site / Route: CA1 field NeuroNames ID : 182
Additional Details: Injected into the ventral hippocampus to target CA1 cells projecting to the medial prefrontal cortex; Forebrain: Hippocampus; Ventral hippocampal CA1 pyramidal neurons projecting to the mPFC

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Virus Name: pENN.AAV.hSyn.Cre.WPRE.hGH
Serotype: AAV Retrograde
Source: Addgene
Catalog Number: 105553-AAVrg
Promoter: Synapsin
Cargo Type: Recombinase
Injection Titer / Dose: 1.20E+13 GC/ml
Injection Volume: 600 nl
Injection Rate: 200 nl/min
Injector Material: Not Provided
Injection Site / Route: Prefrontal Cortex, Neuronames: 1351 Prelimbic area, (area 25 of Brodmann (guenon), Neuronames: 1029
Additional Details: Prelimbic subregion of the medial PFC; Forebrain: Cerebral cortex (neocortex)

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Species: Mouse
Strain or Cell Line: C57/BL6J
Source: Not provided
Other Information: Mutant mice with disruption of BDNF from promoter IV (bred in-house: Maynard et al., https://www.nature.com/articles/npp2015349).
Age at Injection: 3 months
Time After Injection: 6 weeks
Detection Method: Direct fluorescence
Assay & Results: Not Provided
Other Information: No toxicity/inflammation
Tips & Comments: I would use this again for this experiment. Worked well