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pAAV.Syn.GCaMP6s.WPRE.SV40 in Mouse (AAV1)

Addgene #100843-AAV1

Data Submitted By
Heidi Meyer and Francis Lee
Submission Date
July 10, 2019
Publication Date
July 10, 2019 (modified May 16, 2023)
Abstract
Not Provided
Image taken at 10x on a Nikon eclipse fluorescent microscope. Green cells reflect the presence of a GFP (green fluorescent protein variant) attached to the GCaMP molecule.
Caption: Image taken at 10x on a Nikon eclipse fluorescent microscope. Green cells reflect the presence of a GFP (green fluorescent protein variant) attached to the GCaMP molecule.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.Syn.GCaMP6s.WPRE.SV40
Addgene #100843-AAV1

Virus & Injection

Virus

Virus Name
pAAV.Syn.GCaMP6s.WPRE.SV40
Serotype
AAV1
Source
Addgene
Catalog Number
100843-AAV1
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
2.50E+13 GC/mL
Injection Volume
200 nL
Injection Rate
50 nL/min
Injector Material
Not Provided
Injection Site / Route
Hippocampus, NeuroNames ID: 3157
Additional Details
Targeting all neurons in the ventral subregion of the hippocampus.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Jackson Labs
Stock Number
JAX: 000664
Age at Injection
postnatal day 70-90 (adult)
Time After Injection
4 weeks
Detection Method
Direct fluorescence
Assay & Results
Expression was largely localized to the ventral hippocampus. In some cases, expression was also seen in dentate gyrus or entorhinal cortex. Expression was most notable in pyramidal neurons.
Functional Testing
The tool operated as expected. The virus expressed a calcium binding protein bound to a fluorescent reporter (GCaMP). Fluorescence was recorded using fiber photometry, following an emission of 470nm via an optical implant to excite the GCaMP.
Other Information
I did not see toxicity or inflammation
Tips & Comments
No comments