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pAAV.Syn.GCaMP6f.WPRE.SV40 in Mouse (AAV1)

Addgene #100837-AAV1

Data Submitted By
Xiong Xiao and Bo Li
Submission Date
July 09, 2019
Publication Date
July 09, 2019 (modified May 16, 2023)
Abstract
Not Provided

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.Syn.GCaMP6f.WPRE.SV40
Addgene #100837-AAV1

Virus & Injection

Virus

Virus Name
pAAV.Syn.GCaMP6f.WPRE.SV40
Serotype
AAV1
Source
Addgene
Catalog Number
100837-AAV1
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
4.00E+12 GC/ml
Injection Volume
300 nl
Injection Rate
15 nl/min
Injector Material
Not Provided
Injection Site / Route
Solitary nucleus NeuroNames ID : 742
Additional Details
Non-selective neurons in nucleus solitary tract; Nucleus solitary tract; Non-specific neurons in nucleus solitary tract
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Not provided
Age at Injection
8 weeks
Time After Injection
6 weeks
Detection Method
Immunohistochemistry
Assay & Results
Non-specific neurons in nucleus solitary tract.
Other Information
After a long time of expression, there is some toxicity. Try not to extend for 2 months
Tips & Comments
Try to dilute the virus at different concentrations to have the optimal expression in a specific brain region