AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato in Rat (AAV1)
Addgene #51082-AAV1
- Data Submitted By
- Erin Glennon and Robert Froemke
- Submission Date
- July 09, 2019
- Publication Date
- July 09, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: Jasmin Multani
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato
Addgene #51082-AAV1
Virus & Injection
Virus
- Virus Name
- AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 51082-AAV1
- Promoter
- EF1a
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 1.30E+13 GC/mL
- Injection Volume
- two 1 μL injections
- Injection Rate
- 75 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Locus ceruleus, NeuroNames ID: 583
Other Details
- Species
- Rat
- Strain or Cell Line
- LE-Tg(TH-Cre)3.1Deis
- Source
- Rat Resource & Research Center
- Stock Number
- RRRC#: 659
- Age at Injection
- 3-4 months
- Time After Injection
- Checked with fiber photometry from 1 to 5 weeks after injection
- Detection Method
- Immunohistochemistry
- Assay & Results
- Noradrenergic cells of the locus coeruleus. No successful expression.
- Functional Testing
- Attempted to use fiber photometry to detect -- no signal.
- Other Information
- No toxicity, but saw no expression.
- Tips & Comments
- I tested multiple caspids and promoters -- AAV1 and AAV9 don't seem to express well in rat locus coeruleus, but AAV5 does okay. CAG is required for expression.