pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV8)
Addgene #44361-AAV8
- Data Submitted By
- Victor Mathis and Paul Kenny
- Submission Date
- July 09, 2019
- Publication Date
- July 09, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Addgene #44361-AAV8
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-DIO-hM3D(Gq)-mCherry
- Serotype
- AAV8
- Source
- Addgene
- Catalog Number
- 44361-AAV8
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 4.80E+12 GC/ml
- Injection Volume
- 250 nl
- Injection Rate
- 125 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Lateral habenula, NeuroNames ID: 295
- Additional Details
- All cells within the lateral habenula; Forebrain: Epithalamus; Lateral habenula, neurons
Other Details
- Species
- Mouse
- Strain or Cell Line
- Vgat-cre
- Source
- Not provided
- Age at Injection
- 8 weeks
- Time After Injection
- 4 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Not Provided
- Functional Testing
- CNO injected in mice 15 min prior test (3mg/kg). Behavioral response showed reproducibility with previous experiments (electrophysiological recordings in progress)
- Tips & Comments
- For local infusion I would use either an AAV5 or 8