pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 in Mouse (AAV1)
Addgene #98931-AAV1
- Data Submitted By
- Sam Benezra and Randy Bruno
- Submission Date
- July 09, 2019
- Publication Date
- July 09, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40
Addgene #98931-AAV1
Virus & Injection
Virus
- Virus Name
- pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 98931-AAV1
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 7.67E+12 GC/ml
- Injection Volume
- 2 injection sites, 200 nl each site
- Injection Rate
- 30 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Whisker sensory area (Barrel Cortex), NeuroNames ID: 2703
- Additional Details
- Layer 5 neocortex; Forebrain: Cerebral cortex (neocortex); Layer 5 pyramidal neurons in S1 barrel field. This was an RPP4-cre mouse, which sparsely labels layer 5 pyramidal cells
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Not provided
- Age at Injection
- 10 weeks
- Time After Injection
- 3 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- No toxicity or inflammation. The prep lasted over 3 months.
- Functional Testing
- Air puff stimuli were directed at the whisker, which evoked GluSnFR activity in layer 5 apical dendrites.
- Other Information
- No toxicity or inflammation. The prep lasted over 3 months
- Tips & Comments
- No, this virus worked exactly as expected