pAAV.Syn.GCaMP6f.WPRE.SV40 in Mouse (AAV1)
Addgene #100837-AAV1
- Data Submitted By
- Xiong Xiao and Bo Li
- Submission Date
- July 09, 2019
- Publication Date
- July 09, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV.Syn.GCaMP6f.WPRE.SV40
Addgene #100837-AAV1
Virus & Injection
Virus
- Virus Name
- pAAV.Syn.GCaMP6f.WPRE.SV40
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 100837-AAV1
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 4.00E+12 GC/ml
- Injection Volume
- 300 nl
- Injection Rate
- 15 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Anterior insular cortex NeuroNames ID : 3613
- Additional Details
- Not Provided
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Not provided
- Age at Injection
- 8-10 weeks
- Time After Injection
- 5 weeks
- Detection Method
- Immunohistochemistry, In vivo imaging of photometry signals during behavior
- Assay & Results
- Anterior insular cortex (with contamination in somatosensory cortex), without cell-type specificity.
- Other Information
- More toxicity was observed after a very long time (more than 4 months) of expression
- Tips & Comments
- The optimal concentration of virus varies among brain regions. It is important to have a test of injecting various dilutions of virus in a specific brain region