pENN.AAV.hSyn.Cre.WPRE.hGH in Mouse (AAV Retrograde)
Addgene #105553-AAVrg
- Data Submitted By
- Henry Hallock and Keri Martinowich
- Submission Date
- July 09, 2019
- Publication Date
- July 09, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pENN.AAV.hSyn.Cre.WPRE.hGH
Addgene #105553-AAVrg - AAV8.CAG.FLEX.BDNF:HA.WPRE
Virus & Injection
Virus 1
- Virus Name
- pENN.AAV.hSyn.Cre.WPRE.hGH
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 105553-AAVrg
- Promoter
- Synapsin
- Cargo Type
- Recombinase
- Injection Titer / Dose
- 1.20E+13 GC/ml
- Injection Volume
- 600 nl
- Injection Rate
- 200 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Prelimbic area, NeuroNames ID: 1078
- Additional Details
- Targeted the prelimbic subregion of the medial PFC. Injected viruses during the same surgery.
Virus 2
- Virus Name
- AAV8.CAG.FLEX.BDNF:HA.WPRE
- Serotype
- AAV8
- Source
- Unknown
- Catalog Number
- N/A
- Promoter
- CAG
- Injection Titer / Dose
- 4.20E+12 GC/ml
- Injection Volume
- 600 nl
- Injection Rate
- 200 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- CA1 field, NeuroNames ID: 185
- Additional Details
- Targeting ventral hippocampus for expression of BDNF in mPFC-projecting hippocampal neurons in CA1. Injected viruses during the same surgery.
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- N/A
- Other Information
- Mutant mice bred in-house (-e4 mice: Maynard et al., https://www.nature.com/articles/npp2015349).
- Age at Injection
- 3 months
- Time After Injection
- 6 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Ventral CA1 pyramidal neurons. Viruses/titers used worked well.
- Functional Testing
- No tool
- Other Information
- No toxicity/inflammation
- Tips & Comments
- Viruses/titers used worked well