pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Rat (AAV8)
Addgene #44362-AAV8
- Data Submitted By
- Tim O'neal and Susan Ferguson
- Submission Date
- July 05, 2019
- Publication Date
- July 05, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Addgene #44362-AAV8 - CAV2-Cre
Virus & Injection
Virus 1
- Virus Name
- pAAV-hSyn-DIO-hM4D(Gi)-mCherry
- Serotype
- AAV8
- Source
- Addgene
- Catalog Number
- 44362-AAV8
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 2.50E+13 GC/mL
- Injection Volume
- 500 nL
- Injection Rate
- 100 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Nucleus accumbens, NeuroNames ID: 277
- Additional Details
- Targeting nucleus accumbens. Indirect pathway medium spiny neurons of the nucleus accumbens.
Virus 2
- Virus Name
- CAV2-Cre
- Serotype
- N/A
- Source
- Unknown
- Catalog Number
- N/A
- Promoter
- Other
- Other Promoter
- Not Provided.
- Injection Titer / Dose
- 2.50E+09 GC/mL
- Injection Volume
- 500 nL
- Injection Rate
- 100 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Ventral pallidum, NeuroNames ID: 1605
- Additional Details
- Targeting ventral pallidum. Injected 15 min following injection of AAV8-DIO-hM4Di.
Other Details
- Species
- Rat
- Strain or Cell Line
- Wild-type Long Evans
- Source
- Envigo
- Stock Number
- 002
- Age at Injection
- 6 weeks
- Time After Injection
- 3 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Indirect pathway medium spiny neurons of the nucleus accumbens.
- Functional Testing
- Yes - an injection of clozapine-N-oxide (ligand for receptor) but not vehicle had a significant behavioral effect.
- Other Information
- No toxicity or inflammation. Healthy cellular morphology.