pAAV-Syn-ChrimsonR-tdT in Mouse (AAV5)
Addgene #59171-AAV5
- Data Submitted By
- Max Joffe and Jeff Conn
- Submission Date
- July 03, 2019
- Publication Date
- July 03, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pAAV-Syn-ChrimsonR-tdT
Addgene #59171-AAV5 - AAV-CaMKII-ChR2 was injected in a different site (MD thalamus) to examine convergent inputs in the prefrontal cortex.
Virus & Injection
Virus 1
- Virus Name
- pAAV-Syn-ChrimsonR-tdT
- Serotype
- AAV5
- Source
- Addgene
- Catalog Number
- 59171-AAV5
- Promoter
- Synapsin
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 8.20E+12 GC/mL
- Injection Volume
- 400 nL
- Injection Rate
- 100 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Basolateral nucleus of the amygdala, NeuroNames ID: 246
- Additional Details
- Basolateral amygdala pyramidal cells, and their projections to the prefrontal cortex. Basolateral amygdala projection neurons.
Virus 2
- Virus Name
- AAV-CaMKII-ChR2 was injected in a different site (MD thalamus) to examine convergent inputs in the prefrontal cortex.
- Serotype
- N/A
- Source
- Unknown
- Catalog Number
- N/A
- Promoter
- CaMKII
- Injection Titer / Dose
- Unreported GC/mL
- Injection Volume
- 400 nL
- Injection Rate
- 100 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Medial dorsal nucleus, NeuroNames ID : 312
- Additional Details
- AAV-CaMKII-ChR2 was injected in a different site (MD thalamus) to examine convergent inputs in the prefrontal cortex.
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Jackson Labs
- Stock Number
- Jax 000664
- Age at Injection
- 4-6 weeks
- Time After Injection
- 4 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Confirmed red fluorescence in cell bodies in basolateral amygdala injection site and in axon terminals in prefrontal cortex. Then recorded excitatory postsynaptic currents in prefrontal cortex neurons evoked with light stimulation.
- Functional Testing
- Operated as expected.
- Other Information
- Worked well.
- Tips & Comments
- Worked as expected.