pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Mouse (AAV5)
Addgene #44362-AAV5
- Data Submitted By
- Henry Hallock and Keri Martinowich
- Submission Date
- July 02, 2019
- Publication Date
- July 02, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Addgene #44362-AAV5
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-DIO-hM4D(Gi)-mCherry
- Serotype
- AAV5
- Source
- Addgene
- Catalog Number
- 44362-AAV5
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 4.20E+12 GC/mL
- Injection Volume
- 600 nL
- Injection Rate
- 200 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- CA1 field, NeuroNames ID : 182
- Additional Details
- Ventral hippocampus, targeting ventral CA1 Cre-expressing cells from injections of AAVrg-hSyn-EBFP-Cre in the mPFC; Forebrain: Hippocampus; Ventral CA1 neurons, presumably excitatory.
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Jackson Labs
- Stock Number
- JAX: 000664
- Age at Injection
- 3 months
- Time After Injection
- 5 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Expression of mCherry was pretty good.
- Functional Testing
- No tool was used
- Other Information
- No toxicity, no inflammation
- Tips & Comments
- Expression of mCherry was pretty good. I might try a different serotype - hM4Di is tricky to express in subcortical cells