pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV8)
Addgene #44361-AAV8
- Data Submitted By
- Henry Hallock and Keri Martinowich
- Submission Date
- July 02, 2019
- Publication Date
- July 02, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Addgene #44361-AAV8 - AAV8.p4BDNF.ERT2CreERT2.PEST
Virus & Injection
Virus 1
- Virus Name
- pAAV-hSyn-DIO-hM3D(Gq)-mCherry
- Serotype
- AAV8
- Source
- Addgene
- Catalog Number
- 44361-AAV8
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- two 300 nL injections
- Injection Rate
- 200 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Dentate Gyrus, NeuroNames ID: 179
- Additional Details
- Injected into ventral hippocampus - targeting Bdnf promoter IV-expressing cells.
Virus 2
- Virus Name
- AAV8.p4BDNF.ERT2CreERT2.PEST
- Serotype
- AAV8
- Source
- Unknown
- Catalog Number
- N/A
- Promoter
- Other
- Other Promoter
- This virus expresses Cre in promoter IV-expressing cells.
- Cargo Type
- Recombinase
- Injection Titer / Dose
- 2.00E+13 GC/ml
- Injection Volume
- 2 x 300 nL injections
- Injection Rate
- 200 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Dentate Gyrus, NeuroNames ID: 179
- Additional Details
- Same as above - ventral hippocampus.
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Jackson Labs
- Stock Number
- JAX: 000664
- Age at Injection
- 3 months
- Time After Injection
- 4 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Expressed in ventral dentate gyrus, putative excitatory neurons.
- Functional Testing
- Used 4OHT to express Cre, which facilitated expression of hM3Dq-mCherry. Worked as expected.
- Other Information
- No toxicity, no inflammation, worked well.
- Tips & Comments
- Used AAV8 to match serotype of other co-injected virus. Would not change anything.