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pAAV-hsyn-Jaws-KGC-GFP-ER2 in Rhesus macaque (AAV5)

Addgene #65014-AAV5

Data Submitted By
Submission Date
June 27, 2019
Publication Date
June 27, 2019 (modified May 16, 2023)
Abstract
Not Provided
Expression of Jaws on GPi neurons. AAV5-hSyn-Jaws-KGC-GFP-ER2 was injected in the internal globus pallidus (GPi) of a rhesus macaque. The area in the rectangle is shown at higher magnification in the inset. Jaws expression was revealed with antibodies against GFP and immunoperoxidase. GPe, external globus pallidus, OT, optic tract. ((Galvan et al., unpublished data)­
Caption: Expression of Jaws on GPi neurons. AAV5-hSyn-Jaws-KGC-GFP-ER2 was injected in the internal globus pallidus (GPi) of a rhesus macaque. The area in the rectangle is shown at higher magnification in the inset. Jaws expression was revealed with antibodies against GFP and immunoperoxidase. GPe, external globus pallidus, OT, optic tract. ((Galvan et al., unpublished data)­

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-hsyn-Jaws-KGC-GFP-ER2
Addgene #65014-AAV5

Virus & Injection

Virus

Virus Name
pAAV-hsyn-Jaws-KGC-GFP-ER2
Serotype
AAV5
Source
Addgene
Catalog Number
65014-AAV5
Promoter
Synapsin
Cargo Type
Optogenetics
Injection Titer / Dose
3.60E+13 GC/mL
Injection Volume
18 μL
Injection Rate
100 nL/min, increasing to 200 nL/min
Injector Material
Not Provided
Injection Site / Route
Lateral globus pallidus, NeuroNames ID: 232
View in Addgene Catalog

Other Details

Species
Non-Human Primate
Strain or Cell Line
Rhesus macaque
Source
Not provided
Age at Injection
5 years (subject 1); and 5.5 years (subject 2)
Time After Injection
6 to 20 weeks
Detection Method
Immunohistochemistry, Optogenetics
Assay & Results
Trying to target all neurons in Globus pallidus external (GPe ). Jaws expression was revealed with antibodies against GFP and immunoperoxidase. Neurons at injection site (GPe neurons), neurons in neighboring regions (GPi, likely due to spread of virus), and expression of GFP along axons and terminals originating form GPe cells (anterograde transport).
Functional Testing
The opsin Jaws silenced neurons in GPe when activated by light.
Tips & Comments
Inject a smaller volume to keep the expression confined to the region of interest.