pAAV-hSyn-hM4D(Gi)-mCherry in Rat (AAV Retrograde)
Addgene #50475-AAVrg
- Data Submitted By
- Elizabeth Crummy and Susan Ferguson
- Submission Date
- June 21, 2019
- Publication Date
- June 21, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-hSyn-hM4D(Gi)-mCherry
Addgene #50475-AAVrg
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-hM4D(Gi)-mCherry
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 50475-AAVrg
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- two 750 nL injections
- Injection Rate
- 150 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Pontine nuclei, NeuroNames: 617
- Additional Details
- Pyramidal tract cortical neurons.
Other Details
- Species
- Rat
- Strain or Cell Line
- Sprague Dawley
- Source
- Not provided
- Age at Injection
- ~2-3 months old
- Time After Injection
- 2 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Pyramidal tract cortical neurons.
- Target Cell Transduction
- Pyramidal tract neurons in motor, sensory, cingulate, and prelimbic cortices. Some expression in ventroorbital cortex and infralimbic cortex as well.
- Functional Testing
- Yes, there was CNO-induced behavioral changes seen in animals expressing Gi DREADD versus vehicle controls.
- Tips & Comments
- This injection can be very bloody. It may be smart to go at an angle for pons targeting.