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pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Rat (AAV8)

Addgene #44362-AAV8

Data Submitted By: Michael Baratta
Submission Date: June 20, 2019
Publication Date: June 20, 2019 (modified May 16, 2023)
Abstract: Not Provided

DOI:http://dx.doi.org/10.1523/ENEURO.0025-18.2018 Figure 5. "Available for the public to copy, distribute, or display under the Creative Commons Attribution 4.0 International (CC BY 4.0) license." https://creativecommons.org/licenses/by/4.0/ — Caption: DOI:http://dx.doi.org/10.1523/ENEURO.0025-18.2018 Figure 5. "Available for the public to copy, distribute, or display under the Creative Commons Attribution 4.0 International (CC BY 4.0) license." https://creativecommons.org/licenses/by/4.0/
Image attribution: null

Any further use of this image requires permission from the lab who submitted the data.

Vector Used: pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Addgene #44362-AAV8

Virus & Injection

Virus

Virus Name: pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Serotype: AAV8
Source: Addgene
Catalog Number: 44362-AAV8
Promoter: Synapsin
Cargo Type: Chemogenetics
Injection Titer / Dose: 1.90E+13 GC/mL
Injection Volume: 1μL
Injection Rate: 100 nL/min
Injector Material: Not Provided
Injection Site / Route: Prelimbic area, NeuroNames ID: 1078

View in Addgene Catalog

Other Details

Species: Rat
Strain or Cell Line: Sprague-Dawley, female
Source: Not provided
Age at Injection: 9-11 weeks
Time After Injection: 3 weeks
Detection Method: Direct fluorescence
Assay & Results: Prelimbic neurons that project to the dorsal raphe nucleus.
Target Cell Transduction: Yes, expression was restricted to expected pyramidal cell layer.
Other Information: No toxicity was detected