pAAV-hSyn-DIO-mCherry in Rat (AAV8)
Addgene #50459-AAV8
- Data Submitted By
- Michael Baratta
- Submission Date
- June 20, 2019
- Publication Date
- June 20, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
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- Vector Used
-
pAAV-hSyn-DIO-mCherry
Addgene #50459-AAV8
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-DIO-mCherry
- Serotype
- AAV8
- Source
- Addgene
- Catalog Number
- 50459-AAV8
- Promoter
- Synapsin
- Cargo Type
- Control
- Injection Titer / Dose
- 2.10E+13 GC/mL
- Injection Volume
- 1 μL per hemisphere
- Injection Rate
- 100 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Prelimbic area, NeuroNames ID: 1078
Other Details
- Species
- Rat
- Strain or Cell Line
- Sprague Dawley
- Source
- Not provided
- Age at Injection
- 9-11 weeks
- Time After Injection
- 3 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Prelimbic region of the medial prefrontal cortex. Prelimbic neurons that project to the dorsal raphe nucleus. Yes, mCherry expression was restricted to dorsal raphe-projecting prelimbic neurons (Cre-positive). Expression was robust.
- Other Information
- No toxicity was observed, minimal damage surrounding injection site.